Overview
In plain English
The Wolverine Stack is BPC-157 and TB-500 supplied together — 10mg of each in a single vial.
It is the most common pairing in the recovery category. People use it after a specific injury, or through a heavy training block, because the two compounds cover different parts of the repair process rather than doubling up on the same one.
- What it isBPC-157 10mg + TB-500 10mg in one vial
- Mainly used forTendon, ligament and muscle injury recovery
- Typical protocol500mcg of each, once daily, for 4–8 weeks
How it works
What it actually does
Repair needs two things: blood getting to the damaged area, and repair cells physically moving into it.
BPC-157 is associated with the first — new blood vessel growth into tissue that normally has a poor supply. TB-500 is associated with the second — the internal machinery cells use to migrate.
The two do not share a pathway, which is the entire logic of the pairing. No controlled study of the combination itself has been published; the evidence sits with each compound separately.
The science in detail
The rationale for pairing these two compounds is mechanistic separation, not additive potency. They intervene at different points in the tissue repair sequence and, on current evidence, share no common pathway.
BPC-157 is a 15-residue fragment of a protein isolated from human gastric juice. No single high-affinity receptor has been identified for it. Its described actions are extracellular and vascular: Hsieh et al. (Journal of Molecular Medicine, 2017) associated its pro-angiogenic activity with activation and upregulation of VEGFR2 and downstream Akt–eNOS signalling, with the effect attenuated by VEGFR2 blockade. A second body of work describes bidirectional modulation of the nitric oxide system, with Hsieh et al. (Scientific Reports, 2020) reporting effects on vasomotor tone through a Src–caveolin-1–eNOS pathway. In connective tissue, Chang et al. (Journal of Applied Physiology, 2011) reported increased tendon fibroblast outgrowth, survival and migration in vitro. The functional theme is vascular supply and the extracellular signalling environment.
TB-500 is based on thymosin beta-4, the principal G-actin sequestering peptide in mammalian cells. It binds actin monomer in a 1:1 complex and holds it unpolymerised, creating a reservoir that permits rapid, localised F-actin polymerisation at the migrating cell's leading edge. The actin-binding activity localises to the LKKTETQ motif. Malinda et al. (Journal of Investigative Dermatology, 1999) reported accelerated dermal repair in rodent models; Bock-Marquette et al. (Nature, 2004) described a separate pathway through integrin-linked kinase activation in cardiac tissue. A further mechanism operates through liberation of the N-terminal tetrapeptide Ac-SDKP by prolyl oligopeptidase and meprin-α, which carries independent anti-fibrotic activity. The functional theme here is intracellular: cytoskeletal remodelling and cell motility.
One compound addresses whether cells can reach the site and whether it is perfused; the other addresses the machinery cells use to move. That non-overlap is the entire basis of the pairing.
Evidence
What the research shows
Two mechanisms, no overlap
One targets blood supply to the injury, the other targets how repair cells move into it.
Both evidence bases are animal-stage
Each compound has substantial preclinical work behind it and limited human trial data.
No study of the combination
The pairing is reasoned from the separate mechanisms, not from a trial of the two together.
Convenient single vial
One reconstitution, one injection, both compounds at the standard dose.
Mixing & dosage
How to mix and dose it
These are the mixing and dosage instructions supplied by Purist Peptides and the manufacturer for this product.
A 3ml vial of bacteriostatic water is included free with every peptide vial you order, so you have what you need to mix this on arrival.
Wolverine Stack — BPC-157 10mg + TB-500 10mg
Step 1 — mix your vial
- Draw up 2ml of bacteriostatic water into a syringe
- Wipe the rubber top of your vial with an alcohol swab and let it dry
- Insert the needle then slowly and carefully push the water down the inside wall of the vial — do not inject water straight onto the peptide powder
- Gently roll the vial between your palms until the powder dissolves completely
- Do not shake
- Allow to settle in the fridge for 5–10 minutes. The liquid should look clear
Step 2 — draw & inject your dose
Use a 1ml insulin syringe to draw and inject your dose. A 1ml syringe = 100 units.
With 2ml of water added, each 0.1ml (10 units) you draw = 500mcg BPC-157 + 500mcg TB-500.
| Stage | Dose | On the syringe |
|---|---|---|
| Starting | 250mcg each daily | 5 units |
| Standard | 500mcg each daily | 10 units |
Vial duration at standard dose: 20 days
When to inject: Once daily. For acute injury, twice daily — morning and evening.
Cycle length: 4 to 8 weeks, then reassess.
How to inject
- Wipe your injection site with an alcohol swab and let it dry
- Pinch a small fold of skin on your abdomen or inject near the area of concern
- Insert the needle at a 45-degree angle into the pinched skin
- Slowly push the plunger down until empty
- Pull the needle out and apply gentle pressure with the swab
- Dispose of the needle safely in a sharps container — never recap or reuse
Storage
Keep refrigerated at 2 to 6 degrees Celsius at all times after mixing. Do not freeze. Protect from light and label the vial with the date you reconstituted it. Discard any solution that is hazy, discoloured or contains particles.
For informational purposes only. This is not medical advice. Speak to a qualified healthcare professional before starting any compound.
Questions
Frequently asked questions
Is there research on the combination itself?
No controlled study of BPC-157 and TB-500 administered together has been published. The evidence base consists of separate preclinical work on each compound. The pairing is a research convention grounded in mechanistic non-overlap, not a validated protocol, and should be described as such.
Why are these two compounds paired?
Their mechanisms do not intersect. BPC-157's described actions are extracellular and vascular — VEGFR2-associated angiogenic signalling and modulation of nitric oxide. TB-500 acts intracellularly on the actin cytoskeleton, controlling the machinery of cell migration. Pairing them addresses two different constraints on tissue repair.
Do I need to mix the two compounds separately?
No. The Wolverine Stack is supplied as a single pre-mixed vial containing 10mg of each compound, so one reconstitution covers both. Add the bacteriostatic water to the vial as supplied and follow the mixing steps above.
Why is it called the Wolverine Stack?
It is a colloquial name that circulated in research and forum communities, referring to the fictional character's rapid tissue repair. It carries no scientific meaning and implies nothing about the strength of the underlying evidence, which is preclinical.
How is purity verified?
Every batch is assayed by independent HPLC at Bioindustrial Services in Boksburg, and the assay covers both compounds in the vial. We do not rely on the manufacturer's own certificate alone. The assay reports for the batches you receive are available on request, and our full testing protocol is set out at Quality Standards.
How long does delivery take?
Orders are dispatched from South Africa, with timelines varying by region and courier. Current dispatch and transit windows are set out at Delivery Process.
Can I return an order?
Because these are laboratory compounds, returns are not accepted once an order has shipped. If your parcel arrives damaged, contact us within 48 hours with photographs of the packaging and product and we will arrange a replacement. The full terms are set out in our Refund Policy.
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